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Microbial enumeration test

TAMC for bacteria and TYMC for yeast and moulds


Enumeration tests for quantification of microoorganisms in non-sterile products are performed according to Ph Eur 2.6.12 / USP . TAMC (Total Microbial Aerobic Count) is performed for quantification of bacteria and TYMC (Total Yeast and Mould Count) is performed for quantification of yeast and moulds.

Before analysing a product in routine, a Method Suitability Test (MST) is performed. This test verifies the method of choice by pointing out possible antimicrobial properites of the product. Validation is done by inoculating the product with ≤100 CFU of microorganisms, defined by the pharmacopoeia.

The characteristics of the sample/product define which enumeration method that should be used for the test. The methods are described in the Ph Eur / USP:
  • Membrane Filtration: For filterable products, this is the method of choice. Inhibitory substances are removed from the sample by filtration through a 0,45μm filter after which the filter is placed on nutrient media for cultivation.
  • Pour Plate Method: Non-filterable products are moulded together with the nutrient media. Possible inhibitory effects are decreased by diluting the sample.
  • Surface Spread Method: This method has a higher detection limit and is used for non-fiterable samples that cannot be moulded with the media.
  • Most Probable Number Method: This method is used when none of the above techniques can be used and dilution is needed for eliminating inhibitory effects. It is the least sensitive of the methods mentioned.

Analysis result, response time 

Normal time requirement from sample arrival to analysis report-out are 5 to 7 business days but may vary dependent on sample characteristics. For more information, see general terms and conditions.